Extended Data Fig. 3: Negative stain TEM and cryo-EM of CHMP1B/IST1 filaments with different lipid compositions.
From: Brominated lipid probes expose structural asymmetries in constricted membranes
(a) Negative stain TEM micrograph of CHMP1B/IST1 filaments. Black arrows identify examples of vesicular remnants protruding from the ends of filaments, vesicles with no protein bound, or unusually wide filaments. (b) Horizontal slice of a cryo-EM reconstruction of the 17 subunit per turn structure with lipid mixture 1 (c) Vertical slice of a cryo-EM reconstruction of the 17 subunit per turn structure with lipid mixture 1. (d) Horizontal slice of a cryo-EM reconstruction of the 34 subunit per turn structure with lipid composition 3. (e) Vertical slice of a cryo-EM reconstruction of the 34 subunit per turn structure with lipid composition 3. Negative stain micrographs of (f) Lipid composition 1. g) Lipid Mixture 3. (h) Lipid composition 9. (i) lipid composition 2. Cryo-EM micrographs of (j) Lipid composition 1. (k) Lipid composition 3. (l) Lipid composition 9. (m) Lipid composition 2. Scale bars represent 200 nm (a), 10 nm (b–e), and 25 nm (f–m). Negative stain micrographs are representative of approximately 10 recorded for each sample. Cryo-EM micrographs are representative of approximately 500 collected for each sample.
