Fig. 4: Once the structured RNA gets closer to the enzyme, the CSDs reposition dramatically by 70 Å.
a, RNA fold of hairpinD–U7 with a shorter, seven-nucleotide, 3′ overhang. b, Structure of wild-type Dis3L2 in complex with hairpinD–U7. c, A 90° view of b. d, Final 3D map of human Dis3L2 in complex with hairpinD–U7, superimposed with the structure of RNA-free Dis3L2 fit in the map. e, View from the top, showing the change in position of the S1 and CSD domains in the vase and prong conformation. f, Alignment of RNA-free Dis3L2 (gray; vase) and Dis3L2–hairpinD–U7 (colored domains and RNA; prong). The CSD domains are positioned behind S1 in the prong in this view. g,h, Alignment of hairpinA-GCU14 and hairpinD-U7 Dis3L2 structures showing the change in the positioning of the S1 domain (g) and a hairpin in the RNB (residues 555–572) (h).
