Extended Data Fig. 5: Replication forks stall and reverse while facing a transcription-replication conflict (TRC) in Bacillus subtilis (additional biological replicates).
From: Direct visualization of transcription-replication conflicts reveals post-replicative DNA:RNA hybrids
a) EtBr-stained agarose gel of size separated genomic DNA from WT and Δrnhc mutant B. subtilis strains +/− IPTG. Fractions indicated in red were excised for further EM analysis (depicted in Fig. 4d, e)49. b, d-e) and c, f-g) represent two additional biological replicates of the replicate shown in Fig. 4. b, c) EtBr-stained agarose gel of size separated genomic DNA from WT and Δrnhc mutant B. subtilis strains +/− IPTG. Fractions indicated in red were excised for further EM analysis. d, f) Fragment lengths of all imaged RI. The two numbers on top indicate the number of RI within the expected size range (dark dots) and the number of total RI imaged, respectively. e, g) Alignment of selected RI according to daughter strand length. Daughter strands are indicated in green, parental strands in grey. Reversed forks are labeled in pink/black, with pink marking length of the regressed arm and black the length of the parental strand prior to reversal.
