Extended Data Fig. 5: CHK-2 promotes axial cohesin stabilization.
From: ATM signaling modulates cohesin behavior in meiotic prophase and proliferating cells
a, COH-3/4 immunofluorescence in the distal region of gonads, showing that CHK-2 activity controls a cohesin-stabilizing activity that is independently of WAPL-1 suppression. Dashed lines indicate the boundaries between PM and meiotic germline. Scale bar, 10 µM. b, Enlarged images of the regions indicated in a. HTP-3 immunostaining (in magenta) marks chromosome axes. Scale bar, 2 µM. c, Quantification of the intensity of COH-3/4 immunostaining in a and in Fig. 6a. Lower and upper box ends represent the first and third quartiles, with the median indicated as the horizontal line within the box. All data points are shown with the sample sizes indicated below the boxes. ****P < 0.0001 (two-sided Wilcoxon–Mann–Whitney test, adjusted by Bonferroni correction). **P < 0.01 (P = 0.0013, two-sided Wilcoxon–Mann–Whitney test, adjusted by Bonferroni correction) d, WAPL-1 immunostaining in the distal tip of gonads, showing that ATM-1S1853D fails to downregulate the nonphosphorylatable WAPL-12A at meiotic entry. ‘Control’ indicates animals from the same background strain carrying wild-type alleles. Scale bar, 10 µM. e, Quantification of the intensity of WAPL-1 immunostaining in d. Lower and upper box ends represent the first and third quartiles, with the median indicated as the horizontal line within the box. All data points are shown with the sample sizes indicated below the boxes. ****P < 0.0001 (two-sided Wilcoxon–Mann–Whitney test, adjusted by Bonferroni correction). Data for plots in c and e are available as source data.
