Fig. 6: ECO-1-dependent cohesin acetylation contributes to stabilization of axial cohesin against WAPL-1.
From: ATM signaling modulates cohesin behavior in meiotic prophase and proliferating cells
a, ECO-1 is required for robust COH-3/4 localization in EM nuclei if ATM-1-dependent WAPL-1 phosphorylation is defective. Scale bar, 10 µM. b, Enlarged views of the regions indicated in a. Bright HTP-3 staining along axes is detected starting at meiotic entry. Scale bar, 2 µM. d, COH-3/4 immunostaining (in green) of meiotic entry nuclei, showing that the SMC-3 acetylation-mimetic mutation antagonizes WAPL-1-dependent COH-3/4 release upon CHK-2 depletion. Scale bar, 2 µM. c,e, Quantification of the intensity of COH-3/4 immunostaining in b and d, respectively. Lower and upper box ends represent the first and third quartiles, with the median indicated by the horizontal line within the box. All data points are shown, and the sample sizes are indicated. ****P < 0.0001 (two-sided Wilcoxon–Mann–Whitney test, adjusted by Bonferroni correction).
