Extended Data Fig. 5: Steady-state poly(A) length distribution and mRNA abundance changes upon LARPs knockdown.
From: Short poly(A) tails are protected from deadenylation by the LARP1–PABP complex
a. Global poly(A) length distributions of TOP (left) and non-TOP (right) genes in the LARP1 RNA co-IP sample determined by mTAIL-seq. mt-mRNAs were excluded. b.Global poly(A) length distributions of TOP (left) and non-TOP (right) genes in the LARP1 knockdown sample determined by mTAIL-seq. mt-mRNAs were excluded. c. Poly(A) length distributions of mt-mRNAs determined by mTAIL-seq upon depletion of LARP1 or LARP4/4B. d. Global poly(A) length distributions determined by mTAIL-seq upon depletion of LARP4/4B. mt-mRNAs were excluded. The right plot is scaled by the total abundance of mRNAs relative to mt-mRNAs. e. RT-qPCR of the steadily-expressed TOP reporter relative to U1 snRNA upon LARP1 or LARP4/4B depletion (n = 3). The reporter mRNA was targeted by the common constitutive transport element (CTE) in its 3′ UTR. Data are represented as mean ± SEM. P-values (*p < 0.05) were calculated from one-sided Student’s t-test. f. RT-qPCR of the endogenous mRNAs relative to U1 snRNA upon depletion of LARP4/4B measured by qRT-PCR (n = 4). Data are represented as mean ± SEM. P-values (*p < 0.05) were calculated from the one-sided Student’s t-test.
