Extended Data Fig. 5: Germline DNA repair is similar between WT and lin-52 mutant worms and mutant lin-52 alleviates the UV sensitivity of csa-1 but not of xpa-1 mutants.

a, Quantification of CPD nuclei signal intensity in the germline of adult worms irradiated and collected immediately or 60 h after irradiation. The number of nuclei quantified was n = 962, 462, 479, 494 from left to right from 3–5 germlines per strain and condition. The y axis shows the CPD intensity normalized to nuclear DAPI with subtracted background. Box depicts the median with top and bottom quartiles, whiskers to 1.5 IQR. 2-way ANOVA analysis shows a negligible effect of the genotype. b, UV-irradiation assay during somatic development of WT, lin-52(n771), csa-1(tm4539) and lin-52(n771); csa-1(tm4539) mutants. Representative graph showing n = three biological replicates from one out of three independent experiments. Mean +/− SD of each larval stage. Two-tailed t-tests between the fraction of each larval stage of a lin-52; csa-1 compared to csa-1 and lin-52 compared to WT in the same treatment condition are presented. c, UV-irradiation assay during somatic development of WT, lin-52(n771), xpa-1(ok698) and the lin-52(n771); xpa-1(ok698) mutants. Representative graph showing n = three biological replicates from one out of two independent experiments. Mean +/− SD of each larval stage. Two-tailed t-tests between the fraction of each larval stage of a lin-52; xpa-1 compared to xpa-1 and lin-52 compared to WT in the same treatment condition are presented. If P > 0.05, statistics not shown. *P < 0.05, **P < 0.01, ***P < 0.001. Detailed P values and all comparisons against WT and Fisher’s exact tests to compare the population distribution can be found in the Supplementary Table 13.