Extended Data Fig. 7: DREAM complex mutants confer DSB damage resistance and can partially alleviate the sensitivity of DNA repair deficient strains.

a, HRR-dependent IR sensitivity assay in WT and efl-1(se1) mutant. Graph shows the mean of 8 independent experiments, with SEM, each of them with three biological replicates. Two-tailed t-tests between the fraction of surviving embryos within the treatment are presented. b, NHEJ-dependent IR sensitivity assay in WT, lin-52(n771), xpa-1(ok698), polh-1(lf31) and the double mutants lin-52(n771); xpa-1(ok698) and lin-52(n771); polh-1(lf31). Representative graph of one out of three independent experiments, each with n = 3 biological replicates. Mean +/− SD of each larval stage. Two-tailed t-tests between the fraction of the larval stages of lin-52 compared to WT, lin-52; xpa-1 compared to xpa-1 and lin-52; polh-1 compared to polh-1 are presented (showed statistics indicated with upper bracket). c, Lifespan assay upon IR (control and 1000 Gy treatment) of WT, lin-52(n771) and efl-1(se1) mutant worms. log-rang (Mantel-Cox) test was performed to compare the lifespan of the DREAM mutants and WT in the same conditions. Without IR and with IR respectively, n = 62 and 118 for WT, 63 and 116 for lin-52(n771) and 85 and 100 for efl-1(se1). For b, P > 0.05, statistics not shown. *P < 0.05, **P < 0.01. The precise P values and all comparisons to WT worms and Fisher’s exact tests to compare the population distribution can be found in the Supplementary Table 13.