Fig. 4: Mutations in the DREAM complex confer DNA-damage resistance against multiple damage types.

a, IR sensitivity dependent on HRR was tested in WT, lin-52(n771), HRR-deficient brc-1(tm1145); brd-1(dw1), NHEJ-deficient cku-70(tm1524), lin-52(n771); brc-1(tm1145); brd-1(dw1), lin-52(n771); brc-1(tm1145); brd-1(dw1); cku-70(tm1524) and lin-52(n771); cku-70(tm1524) worms. Data are shown as mean ± s.e.m., n = 4 independent experiments (n = 3 for lin-52; cku-70). Each independent experiment had three biological replicates. Two-tailed t-tests were used for statistical comparisons, and the most relevant comparisons are shown. b, IR sensitivity assay dependent on NHEJ repair in WT, lin-52(n771), NHEJ-deficient cku-70(tm1524) and lin-52(n771); cku-70(tm1524) worms. The graph is representative of n = 3 biological replicates in 1 of 3 independent experiments, each of which had 3 biological replicates. Data are shown as mean ± s.d. of each larval stage. Two-tailed t-tests were used to compare the fraction of the larval stages of lin-52 compared with WT, and lin-52; cku-70 compared with cku-70 (non-significant). c, Alkylation-damage assay of WT, lin-52(n771), alkylation-damage-sensitive polh-1(lf31) and double-mutant lin-52(n771); polh-1(lf31) worms. The graph is representative of n = 3 biological replicates in 1 of 3 independent experiments. Data are shown as mean ± s.d. of each larval stage. Two-tailed t-tests were used to compare the fraction of the larval stages of lin-52 with WT, and lin-52; polh-1 with polh-1. d, ICL assay of WT and lin-52 mutants upon cisplatin treatment. Because cisplatin was diluted in DMF, worms were also given the maximum dose of DMF that was given for the cisplatin treatments as additional control. The graph is representative of n = 3 biological replicates in 1 of 3 independent experiments. Data are shown as mean ± s.d. of each larval stage. Two-tailed t-tests were used to compare the fraction of the larval stages of lin-52 compared with WT. P > 0.05, not shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Detailed P values and more comparisons can be found in Supplementary Table 13, including results from Fisher’s exact test, which was used to analyze the distribution of the larval stages.