Fig. 6: Esrrb association with euchromatin and heterochromatin regions during mitosis. | Nature Structural & Molecular Biology

Fig. 6: Esrrb association with euchromatin and heterochromatin regions during mitosis.

From: Loss of H3K9 trimethylation alters chromosome compaction and transcription factor retention during mitosis

Fig. 6: Esrrb association with euchromatin and heterochromatin regions during mitosis.

a, Representative images of Esrrb–tdTomato ESC metaphase spreads stained with DAPI (blue). Scale bar, 10 μm. b, Esrrb immunolabeling (red) of flow-sorted chromosomes 19 (top panel) and X (lower panel), where DAPI counterstain is shown in light gray. Scale bar, 5 μm. Linescan analysis (profile plots) is shown of Esrrb (red) and DAPI (gray) intensities across chromosomes 19 and X (right panels). Ab, antibody. c, Representative fluorescence images of Esrrb–tdTomato ESCs at metaphase (left) and telophase (right) stages with and without chaetocin treatment. DAPI stain is in blue. d, Cot-1 (pink) and γSat repeat (yellow) RNA–FISH in WT and Suv39h dn ESCs, with DAPI stain in blue. Scale bar, 5 μm. All the images represent three independent experiments.

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