Table 1 Cryo-EM data collection statistics
Bead (EMDB-13984) | Arm (EMDB-13986) | |
|---|---|---|
Magnification | 64,000× | 64,000× |
Voltage (kV) | 300 | 300 |
Electron exposure (e− Å−2) | 66 | 66 |
Defocus range (μm) | 2–5 | 2–5 |
Pixel size (Å) | 2.22 | 2.22 |
Symmetry imposed | C2 | C2 |
Initial particle images | 27,737 | 27,737 |
Final particle images | 7,139 | 4,957 |
Map resolution (Å) | 9.7 | 18.3 |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 4.6.0–36.7 | 13.6–24.2 |