Extended Data Fig. 6: Comparison of SthK with CNG and HCN channels, with a focus on their cN binding pockets.
From: Discrimination between cyclic nucleotides in a cyclic nucleotide-gated ion channel
Top: cNs binding pocket of SthK (left, gray) and CNGA1 (right, orange) showing that the residues Y and F are swapped in CNGA1 channels compared with SthK. Bottom: Sequence alignment of SthK with CNG and HCN channels showing that Y357 and F365 identified by MDS to be crucial in cN discrimination in SthK are not conserved, whereas M369 is a conservative mutation and A370 is a semi-conservative mutation. cAMP interacts with Y357, M369 and A370, and cGMP interacts with F365.
