Extended Data Fig. 1: cNs-CNBD binding studies using microscale thermophoresis (MST).
From: Discrimination between cyclic nucleotides in a cyclic nucleotide-gated ion channel
a) and c) Microscale thermophoresis (MST) traces of cAMP-CNBD (a) and cGMP-CNBD (c) interactions, respectively. b) Binding curve of labeled CNBD with cAMP. Two binding phases were detected. The first had a KD of 0.4 ± 0.5 µM, and the second had a KD of 1.6 ± 1.1 µM. d) Binding curve of labeled CNBD with cGMP. The KD value of cGMP-CNBD was 3.3 ± 1.8 µM. The concentration of labeled His6-C-linker-CNBD was kept constant (50 nM), and the concentration of cNs was varied from 0.00305 µM to 100 µM.
