Extended Data Fig. 2: Distributions of rupture forces of cAMP-CNBD or cGMP-CNBD bonds under various conditions.
From: Discrimination between cyclic nucleotides in a cyclic nucleotide-gated ion channel
a) Force distribution of cAMP-CNBD unbinding following 0.02 s bond formation at varying pulling velocity (top right in each graph). b) Force distribution of cAMP-CNBD unbinding following 1.00 s bond formation at varying pulling velocities (top right in each graph). The force distributions are fitted with bimodal Gaussian fits to extract the most probable rupture forces for binding state 1 (first peak) and binding state 2 (second peak), which are then used for Bell–Evans model fitting. c) Force distribution of cGMP-CNBD unbinding following 0.02 s bond formation at varying pulling velocities (top right in each graph). d) Force distribution of cGMP-CNBD unbinding following 1.00 s bond formation at varying pulling velocities (top right in each graph). e) and f) Distributions of rupture forces of cGMP-CNBD (e) and cAMP-CNBD (f) unbinding following different bond formation times and at 0.4 µm/s pulling velocity). Bimodal Gaussian fits are used to extract the number of events for each bond state, which is then used to calculate the probability of occurrence of binding state 2.
