Extended Data Fig. 10: Control experiments for the glycerol density gradient sedimentation analysis of RecFOR proteins in the presence of 3′ overhang dsDNA.
From: Mechanism of RecF–RecO–RecR cooperation in bacterial homologous recombination
Silver-stained SDS-PAGE analysis of the fractions from the control experiments are shown. Fractions from low to high molecular weight were analyzed and their numbers are given on the top of the gels. The proteins used in each experiment are indicated on the left of each gel. Each experiment was repeated three times. ‘M’ lane shows the loading control. The gel at the top shows standard protein ruler and loading control. RecF, RecO and RecR proteins were applied in 2:1:4 molar ratio in loading controls.
