Extended Data Fig. 5: Cryo-EM analysis of the yeast Rpd3L complex.

a, The final reconstruction for the Rpd3L complex displays an average resolution of 2.6 Å on the basis of the Fourier-shell correlation (FSC) value of 0.143. b, Two overall views of the EM density map. The local resolutions are color-coded for different regions of Rpd3L complex. c, Angular distribution of the particles used for the reconstruction. d, The FSC curves for cross-validation between the model and the cryo-EM maps of yeast Rpd3L complex. Shown here are the FSC curves between the final refined atomic model and the reconstruction from all particles (black), between the model refined in the reconstruction from only half of the particles and the reconstruction from that same half (red), and between that same model and the reconstruction from the other half of the particles (green).