Extended Data Fig. 6: HPF1 regulates the recruitment of DNA-binding repair factors to sites of damage.

(a) Immunoflurosecence showing depletion of HPF1 in ZNF384^KO cells. Hoechst staining shows nuclei. Scale bar, 10 μm. (b-d) Representative confocal images (b), recruitment kinetics (c), and mean recruitment intensity at 200 s post-irradiation (d) of GFP-E4F1 at sites DNA damage induced by 405 nm irradiation in U2OS WT and HPF1^KO cells treated or not with PARPi. Confocal images are 200 s post-irradiation. Scale bar, 5 μm. Recruitment kinetic curves show the mean ± SEM Data from c,d are a representative of 3 independent replicates where data were collected from 11–13 cells per condition. (e, f) Representative confocal images (e) and recruitment kinetics (f) of GFP-CHD4 at sites DNA damage induced by 405 nm irradiation in U2OS WT, PARP1^KO and HPF1^KO cells. Confocal images are 200 s post-irradiation. Scale bar, 5 μm. Recruitment kinetic curves show the mean ± SEM. Data from f are a representative of 3 independent replicates where data were collected from 13–22 cells per condition. (g, h) Representative confocal images (g) and recruitment kinetics (h) of GFP-CHD7 at sites DNA damage induced by 405 nm irradiation in U2OS WT, PARP1^KO and HPF1^KO cells. Confocal images are 200 s post-irradiation. Scale bar, 5 μm. Recruitment kinetic curves show the mean ± SEM. Data from h are a representative of 3 independent replicates where data were collected from 13–16 cells per condition.

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