Extended Data Fig. 7: HPF1-dependent recruitment of DNA-binding repair factors to sites of damage is promoted by histone ADP-ribosylation.

(a–c) Representative confocal images (a), recruitment kinetics (b), and mean recruitment intensity at 200 s post-irradiation (c) of GFP-E4F1 at sites DNA damage induced by 405 nm irradiation in U2OS PARP1^KO cells complemented or not with mCherry-PARP1 WT, PARP1 3SA or PARP1 LW/AA. Confocal images are 200 s post-irradiation. Scale bar, 5 μm. Recruitment kinetic curves show the mean±SEM. Data from b,c are a representative of 3 independent replicates where data were collected from 12–16 cells per condition. (d-f) Representative confocal images (d), recruitment kinetics (e), and mean recruitment intensity at 200 s post-irradiation (f) of GFP-CHD4 at sites DNA damage induced by 405 nm irradiation in U2OS PARP1^KO or PARP1/HPF1 double knockout cells complemented or not with mCherry-PARP1 WT, PARP1 3SA or PARP1 LW/AA mutants. ∅ denotes no plasmid expression. Confocal images are 200 s post-irradiation. Scale bar, 5 μm. Recruitment kinetic curves show the mean ± SEM. Data from e,f are a representative of 3 independent replicates where data were collected from 10–13 cells per condition. (g-i) Representative confocal images (g), recruitment kinetics (h), and mean recruitment intensity at 200 s post-irradiation (i) of GFP-CHD7 at sites DNA damage induced by 405 nm irradiation in U2OS PARP1^KO or PARP1/HPF1 double knockout cells complemented or not with mCherry-PARP1 WT, PARP1 3SA or PARP1 LW/AA mutants. ∅ denotes no plasmid expression. Confocal images are 200 s post-irradiation. Scale bar, 5 μm. Recruitment kinetic curves show the mean ± SEM. Data from h,i are a representative of 3 independent replicates where data were collected from 10–12 cells per condition.

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