Extended Data Fig. 1: SENP1 negatively regulates RLR signaling.
From: MAVS deSUMOylation by SENP1 inhibits its aggregation and antagonizes IRF3 activation
(a) IB of SENP1 knockdown efficiency by Senp1 shRNAs (sh-Senp1 no. 1 to no. 4). (b) IFN-β-Luc (left) and PRD I-III Luc (right) activity analysis of RAW264.7 cells transfected with Co.sh or sh-Senp1, followed by infection with or without SeV for 12 h. (c) qPCR of Ifnb1 mRNA in RAW264.7 cells transfected with Co.sh or sh-Senp1 and treated with SeV for indicated time points. Senp1 knockdown efficiency is shown on the left panel. (d) qPCR of IFNB1 mRNA in HEK293T cells transfected with Co.sh or SENP1 shRNA (sh-SENP1) and treated with SeV for indicated time points. SENP1 knockdown efficiency is shown on the left panel. (e) IB of HEK293T cells transfected with Co.vec, SENP1 wt, or SENP1 mt. (f) IFN-β-Luc (left) and PRD I-III Luc (right) activity analysis of HEK293T cells transfected with Co.vec, SENP1 wt, or SENP1 mt, followed by infection with or without SeV for 12 h. (g) qPCR of IFNB1 mRNA in HEK293T cells transfected with Co.vec, SENP1 wt, or SENP1 mt, followed by SeV infection (left) or poly (I:C) LMW transfection (right) for various time. (h) qPCR of Ifnb1 mRNA in RAW264.7 cells transfected with Co.vec, SENP1 wt, or SENP1 mt, followed by SeV infection (left) or poly (I:C) LMW transfection (right) for various time. b-d, f-h, n = 3 biological independent samples; data are presented as mean ± s.d.; statistical analyses were performed using a two-tailed Student’s t-test. IB=immunoblot, Co.sh=control shRNA, Co.vec=control empty vector, wt=wild-type, mt=mutant.
