Extended Data Fig. 9: L1-hairpin-mut AtAgo10 is impaired in target-slicing but not target-binding.

a. Blots from an RNA filter-binding experiment using L1-hairpin-mut AtAgo10 (10 nM) and target RNAs (2 nM) used in this study (shown in Fig. 4b). Complexes were formed with ³²P-labeled target RNAs under conditions used for slicing experiments (except Mg²⁺ was omitted to prevent target cleavage) and passed through a nitrocellulose membrane (to capture protein-RNA complexes) followed by a nylon membrane (to capture RNAs not retained on the nitrocellulose). b. Quantitation of data in panel a. Plotted data are the values from n = 1 experiments. c. Fraction of 2–21 target RNA (2 nM) cleaved by wild-type (10 nM) or L1-hairpin-mut AtAgo10 (10 nM) versus time. Data points are the mean values of n = 3 independent experiments. Error bars indicate SEM.