Extended Data Fig. 1: RNC-Sec61-TRAP complex assembly.
From: Molecular basis of the TRAP complex function in ER protein biogenesis
The complex was assembled using stalled RNCs carrying an ER client mixed with canine SRP and salt- and EDTA-treated canine ER microsomes (EKRM). Microsomes were first pelleted then solubilized with 2% digitonin. Ribosome-bound complexes were then pelleted over a sucrose cushion and applied directly to cryo-EM grids.
