Fig. 3: Protein fragment activity in the tethering screen represents real, verifiable regulatory function.
From: Surveying the global landscape of post-transcriptional regulators
a, Distribution of sequencing reads across subpopulations separated by FACS. b, Median activity of each protein fragment in the flow cytometry tethering assay (n = 2 per fragment). c, Comparison of the log2(difference in fluorescence ratio) and the screen activity score per fragment, r = 0.91. d, Flow cytometry measuring activity of Sbp1 and Sbp1(14–178) in the tethering assay (n = 2, one replicate per sample is shown). e, As in d, for Sro9 and Sro9(14–151) (n = 2, one replicate per sample is shown). f, As in d, for Jsn1 and Jsn1(144–295) (n = 2, one replicate per sample is shown). g, As in d, for Yap1801 and Yap1801(374–527) (n = 2, one replicate per sample is shown).
