Extended Data Fig. 3: Vanadate-mediated photocleavage assay.
(a) Schematic depicting expected vanadate-mediated photocleavage if vanadate were bound to AAA1 (top) or AAA3 (bottom). (b and c) Two independent replicates of photocleavage assay. Purified indicated yeast dyneinMOTOR fragments were incubated with 3 mM ATP with or without 3 mM vanadate, exposed to ultraviolet light (254 nm) for 1 hour, and then analyzed by coomassie-stained SDS-PAGE.
