Extended Data Fig. 4: Cryo-EM data processing flowchart. | Nature Structural & Molecular Biology

Extended Data Fig. 4: Cryo-EM data processing flowchart.

From: Microtubule-binding-induced allostery triggers LIS1 dissociation from dynein prior to cargo transport

Extended Data Fig. 4: Cryo-EM data processing flowchart.

(a) Cryo-EM workflow of MT-B in the presence of ATP (details in Methods). (b) FSC curves with the gold standard threshold of 0.143 for MT-B. (c, d) Particle distribution plot and 3D FSC analysis of MT-B. (e) Local resolution analysis of MT-B and representative cryo-EM densities. (f) Cryo-EM workflow of MT-U with LIS1. (g) FSC curves with the gold standard threshold of 0.143. (h, i) Particle distribution plot and 3D FSC analysis of MT-U + LIS1 in the presence of ATP and Vi. (j) Local resolution analysis and representative cryo-EM densities of the LIS1-LIS1 interface. (k) Cryo-EM workflow of MT-U alone in the presence of ATP and Vi. (l) FSC curves with the gold standard threshold of 0.143. (m, n) Particle distribution plot and 3D FSC analysis of MT-U. (o) Local resolution analysis and representative cryo-EM densities.

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