Extended Data Fig. 5: rGAT1EM-Fab epitope and transporter densities.
From: Cryo-EM structure of GABA transporter 1 reveals substrate recognition and transport mechanism
a, Epitope interactions of rGAT1EM with the variable regions of heavy and light chains 9D5 modeled into the density and the epitope mutant residues are fit into unambiguous densities in the map modeled at 6.0σ. b, Transmembrane helices TM1-12 and extracellular loop2 of rGAT1EM fit into cryo-EM density map contoured at a level (σ) of 4.5 display clear presence of side chain densities, disulfide bond and N-glycosylation sites.
