Extended Data Fig. 2: Foci images of in vitro replication kinetics of the authentic viruses.
From: Structural and functional characteristics of the SARS-CoV-2 Omicron subvariant BA.2 spike protein
(a) and (b) Vero E6-TMPRSS2 cells were infected with the authentic G614 (B.1), Delta (B.1617.2), Omicron-BA.1 or BA.2 viruses at MOI of 0.005. Postinfection supernatants were titrated by a focus-forming assay. Foci as a cluster of cells expressing viral antigen were imaged and counted using AID vSpot Spectrum. (a) Extended virus replication kinetics in Vero E6-TMPRSS2 cells. Focus-forming units (FFU) per ml were determined and data are expressed as mean ± sem of n = 3 independent replicates/time point/virus. (b) images of foci (dark spots) titration shown in (a) with automatic counts in the lower corner of each well. (c) Vero E6 cells cells were infected with the authentic G614 (B.1), Delta (B.1617.2), Omicron-BA.1 or BA.2 viruses at MOI of 0.005. The data are summarized in Fig. 1d. (c) Vero E6-TMPRSS2 cells were infected with the authentic G614 (B.1), Delta (B.1617.2), Omicron-BA.1 or BA.2 viruses at MOI of 0.005. The data are summarized in Fig. 1e.
