Extended Data Fig. 3: Infection of HEK293-ACE2 cells by MLV-based pseudotyped viruses.
From: Structural and functional characteristics of the SARS-CoV-2 Omicron subvariant BA.2 spike protein
Time course for single-cycle infection of HEK293-ACE2 cells by MLV-based pseudotyped viruses with various SARS-CoV-2 variant S constructs, as indicated, all containing a CT deletion. Infection was initiated by mixing viruses and target cells, and viruses were washed out at each time point as indicated. Delta AY.4.2 is a Delta subvariant. The relative light unit (RLU) of 8-hr infection of each pseudovirus was used as 100% infectivity for normalizing data at other time points. The relative infectivity (%) equals RLU(t)/RLU(8-hr)*100. The experiments were repeated at least three times with independent samples giving similar results. Error bars are standard deviations of 4 repeats of each data point (n = 3).
