Fig. 3: Endogenous TFIID subunits are localized in physical proximity to TAF1 mRNA in the cytoplasm of human cells.
From: Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID
a, Schematic overview of the imaging strategy used to detect co-TA events of endogenous TFIID subunits on TAF1 mRNA with a combination of smFISH and IF. b–f, Representative multicolor confocal images from the co-localization assay shown in a for each assessed subunit. Each image is a single multichannel confocal optical slice. Protein IF and TAF1 mRNA detection are shown in green and magenta, respectively. Co-localizing spots are indicated with yellow arrows. Zoomed-in regions (white squares) are shown below each image. Inset scale bars, 1 μm. The plots on the right report the fraction of target mRNAs co-localized with protein signal for each experimental condition. Each open circle corresponds to an independent field of view (n = 3, total number of cells is in brackets).
