Fig. 4: The cytoplasm is populated by multisubunit TFIID ‘building blocks’.
From: Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID
a–f, IP of endogenous TFIID subunits coupled to label-free MS, performed on cytoplasmic extracts from human HeLa cells. Bar plots represent the average NSAF (normalized spectral abundance factor) value for each detected subunit in technical triplicates. Error bars represent the s.e.m. The antigen position of the TAF1 antibody used for IP is shown in f. g, Visual summary of cytoplasmic TFIID submodules inferred from IP–MS data. IPs in which the given submodule was enriched are indicated.
