Fig. 6: TAF1 depletion leads to an accumulation of TFIID building blocks in the cytoplasm.
From: Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID
a, Subcellular fractionation of siRNA-transfected HeLa cells followed by western blot analysis of endogenous TFIID subunits distribution. GAPDH, lamin A/C and histone H3 were used as loading controls. The amount of loaded cytoplasmic (cyto.) extract is three times the amount of the nuclear (nucl.) extract counterpart. The positions of the molecular weight markers in kDa are indicated on the right. b, IP of endogenous TFIID subunits coupled to label-free mass-spectrometry (MS) performed on cytoplasmic extracts of siRNA-transfected HeLa cells. The circle area represents the average NSAF value for each detected subunit in technical triplicates. The NSAF scales are indicated on the right. Distinct TFIID subcomplexes are depicted on the top. Subunits are color-coded and arranged according to the subcomplexes indicated on the top. Black dots in the circles identify the protein used as bait in each IP. c, Same as in b, but the IPs were performed on nuclear extracts. CTR, non-targeting control siRNA. Co., complex.
