Extended Data Fig. 2: TAF1 smFISH probes validation.
From: Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID
a, TAF1 siRNA-mediated KD assessed with RT-qPCR and expressed relative to control siRNA (CTR). Data points correspond to four biological replicas. Data presented as mean value +/− SD. b, Representative confocal maximum intensity projections (MIPs) of TAF1 smFISH on HeLa cells transfected with control siRNA (siCTR) or siRNA directed against TAF1 (siTAF1). smFISH and DAPI channels are displayed using the green fire blue and grayscale color scales, respectively. c, TAF1 KD quantification. Violin plot representing the absolute number of cytoplasmic mRNAs per cell (total number of analyzed cells is in brackets). The boxplot shows the median, 25th and 75th percentile box bounds, and whisker limits as 1.5× interquartile range.
