Extended Data Fig. 2: Characterization of the erythroid transcriptome.
From: RNA polymerase II pausing regulates chromatin organization in erythrocytes
a, Spearman correlation heatmap of RNA-seq replicates. b, Venn diagram of expressed genes in fibroblasts and erythroid cells. c, Relative expression of GAPDH and HBBA measured by RT-qPCR, triplicates from two biological replicates are presented. For aRBC and fibroblasts this means independent samples from two different animals, for eRBC this means two independent RBC collection from approximately 20 embryos, and for erythroblasts this means two independent cell cultures. Data is presented as means +/− SD. P values indicated are based on one-way ANOVA analysis followed by Tukey’s post-hoc test d, Expression levels of the erythroid genes EPB41 and EPB42 measured by RNA-seq (up; n = 3 independent RNA-seq libraries) and RT-qPCR (down: n= same as c). Data is presented as means +/− SD with P values based on one-way ANOVA followed by Tukey’s post-hoc test. e, EU incorporation measured by fluorescence intensity after 6 hours of incubation with EU (n = 50 nuclei from three independent Click-iT experiments). P values based on one-way ANOVA analysis followed by Kruskal-Wallis post hoc test are presented. f, MA plots of differentially expressed genes between eRBC and erythroblasts, and aRBC and erythroblasts. g, GO terms enriched in upregulated genes from f. Analysis was performed using Fisher’s exact test and the default multiple-hypotheses testing method (g:SCS) on gProfiler.
