Extended Data Fig. 1: Cryo-EM processing workflow, showing local and global map quality for the Oat1–α-ketoglutarate sybody complex.
From: Molecular basis for selective uptake and elimination of organic anions in the kidney by OAT1
a, Image processing workflow for Oat-sybody with α-KG. b, Gold-standard FSC curve used for global-resolution estimates within cryoSPARC. c, Local-resolution of reconstructed map as determined within cryoSPARC. Detergent density omitted for clarity. Sybody density is shown in wheat, Oat1 density blue and nonprotein density gray. d, Close-up view of map and side-chain density for transmembrane helices. e, Density for α-KG and side-chains coordinating the molecule. Density colour is the same resolution range given in c.
