Extended Data Fig. 5: Cryo-EM processing workflow, showing local and global map quality for the low occupancy Oat1–α-ketoglutarate sybody complex.
From: Molecular basis for selective uptake and elimination of organic anions in the kidney by OAT1
a, Image processing workflow for Oat-sybody with low occupancy α-KG. b, Gold-standard FSC curve used for global−resolution estimates within cryoSPARC. c, Local-resolution of reconstructed map as determined within cryoSPARC. Detergent density omitted for clarity. d, Density for TM8 and high occupancy α-KG from PDB 8BW7. Inset zoomed in view of density showing interaction Lys382 and the keto group of α-KG. e, Density for TM8 and low occupancy α-KG from PDB 8OMU. The α-KG molecule from d is shown for comparison. Inset zoomed in view of α-KG binding site (site 1) showing weak density for the ligand. f, and g, zoomed in views of TM8 from d and e respectively.
