Extended Data Fig. 4: AlphaFold2-predicted models of DELE1 and negative stain EM.
From: DELE1 oligomerization promotes integrated stress response activation
a, The AlphaFold2 predicted structure with the DELE1 CTD sequence harboring L239E, L242E, F250A, L251E, R403A, and F431S mutations. The mutated residues are denoted as yellow spheres. b, Structural alignment of the DELE1 cryo-EM structure and AlphaFold2-predicted monomeric structure in a, showing that the overall predicted structure of the mutant DELE1 is highly similar to the WT DELE1. c-e, Representative negative stain EM images of the WT DELE1, the Interface II mutant (R403A/F431S), and the Interface I truncation (Δ244). A total of 326, 257, and 392 images were collected, respectively. f, Fluorescence-detection size-exclusion chromatography (FSEC) analysis for WT DELE1CTD-GFP (labeled as WT in blue) and DELE1CTD-GFP Δ244 truncation (labeled as Δ244 in magenta) expressed for 24 hrs post transfection from HEK293T cells.
