Extended Data Fig. 4: Interface of bnDs and Env.
From: Trapping the HIV-1 V3 loop in a helical conformation enables broad neutralization
A) Interface of bnD.9 and V3 on BG505.SOSIP.664. Residues within 4 Å distance between bnD.9/Env are labeled and shown as sticks. B) Interface of bnD.9 and bridging sheet. Residues within 4 Å distance between bnD.9/Env are labeled and shown as sticks. C) Buried surface Area (BSA) of V3 residues and additional sites in β20-β21 and the V1V2-base in bnD.8 and bnD.9 X-ray structures in complex with V3 peptides and bnD.9 cryo-EM structure in complex with BG505.SOSIP.664 and sCD4. The % BSA was calculated as the ratio of BSA to accessible surface area. D) Ligplot depiction of DARPin/Env interface in X-ray structures of bnD.8 and bnD.9. DARPin residues are shown in blue, Env residues in orange. Eyelashes indicate a hydrophobic interaction, dotted green lines hydrogen bonds.
