Fig. 3: V3-CD4i bnDs are highly dependent on Env opening and act predominantly after attachment.
From: Trapping the HIV-1 V3 loop in a helical conformation enables broad neutralization
a, Effect of trimer opening on the neutralization potency of bnD.8 and bnD.9. Single JR-CSF alanine mutants known to confer trimer opening were compared for sensitivity to bnDs and antibodies. Bars indicate fold change of IC50 relative to wild-type JR-CSF; depicted ratios are means from two to four independent experiments. mut, mutant; wt, wild-type. b, Preattachment and postattachment inhibition activity of bnDs and bnAbs against BG505 and SF162 pseudoviruses as deferred in an adapted TZM-bl pseudovirus assay where total activity corresponds to the conventional neutralization setup with inhibitor present at all steps and consisting of an activity before attachment and after attachment. Through measuring postattachment activity in a setup where inhibitors are added after virus binding to cells, preattachment activity can be deferred. This total activity measured at a fixed antibody (6.4 nM) or bnD (10 µM) concentration is set to 100%, and preattachment and postattachment activity are calculated relative to it. Values are means of three or four independent experiments.
