Extended Data Fig. 2: Alignment of V3 dependent bnDs.
From: Trapping the HIV-1 V3 loop in a helical conformation enables broad neutralization
Amino acid sequence alignment of V3 bnDs selected in selections I-V (bnD.4 and bnD.8 to bnD.15) and in published V3 bnDs (bnD.1 to bnD.320,. Alignment of V3-crown (bnD.1-bnD.4) and V3-CD4i bnDs (bnD.8-bnD.15). The library sequence indicates randomized residues by “X”, mutated residues in the respective DARPins are indicated by colors. All bnDs are N3C DARPins except bnD.2 and bnD.4 which are N2C DARPins lacking one internal repeat, indicated by dashes.
