Extended Data Fig. 1: Protein Degradation Defects in the pre1-1, 4-1 Mutant.
From: Structure of the preholoproteasome reveals late steps in proteasome core particle biogenesis
The rate of degradation of a short-lived cytoplasmic protein (Tmc1; panel a) and an ERAD substrate (CPY*; panel b) was determined by cycloheximide chase assay. Cycloheximide (100 μg/ml) was added at time zero, and whole cell extracts were prepared at the indicated time points. SDS-PAGE was performed, followed by immunoblot analysis with antibodies against Tmc1, CPY*, and Hog1 (loading control). Similar results were seen in two experiments for each of the two substrates.
