Extended Data Fig. 7: Analysis of mRNA enrichment using short isoforms.
From: m6A governs length-dependent enrichment of mRNAs in stress granules
a-b, Cumulative distribution plots of differential expression using shortest isoforms. Data analysis and interpretation is essentially the same as Fig. 4c,d. c-d, Box plots of differential expression using shortest isoforms. Data analysis and interpretation is essentially the same as Extended Data Fig. 5a,b. Boxplots depict the median (heavy line), upper and lower quartile medians (box), and interquartile range (whiskers, IQR = 1.5). Outliers are plotted separately. For c, control: n = 13311 transcripts; 0-0.5 kb, n = 1450; 0-1 kb, n = 2889; 1-1.5 kb, n = 1404; 1.5-2 kb, n = 1475; 2-2.5 kb, n = 1246; 2.5-3 kb, n = 1121; 3-4 kb, n = 1593; 4-5 kb, n = 933; 5-6 kb, n = 477; 6+ kb, n = 723) and for d, Mettl3 KO (n = 13511 transcripts; 0-0.5 kb, n = 1449; 0-1 kb, n = 2923; 1-1.5 kb, n = 1422; 1.5-2 kb, n = 1500; 2-2.5 kb, n = 1270; 2.5-3 kb, n = 1148; 3-4 kb, n = 1627; 4-5 kb, n = 949; 5-6 kb, n = 485; 6+ kb, n = 739).
