Extended Data Fig. 10: mRNA enrichment as a function of transcript length and m6A in NIH3T3 cells.
From: m6A governs length-dependent enrichment of mRNAs in stress granules
a, Boxplots demonstrating the correlation between m6A levels and stress granule enrichment in NIH3T3 cells. The y-axis shows the level of enrichment of transcripts in stress granules relative to the cytoplasm. The x-axis shows the number of m6A sites per transcript. Boxplots depict the median (heavy line), upper and lower quartile medians (box bounds), and interquartile range (whiskers, IQR = 1.5) Each dot represents a unique individual transcript. n = 8301 transcripts; 0 m6A sites, n = 5607; 1 m6A site, n = 1015; 2 m6A sites, n = 1023; 3 m6A sites, n = 300; 4 + m6A sites, n = 356. At the top of each boxplot the range of transcript lengths contained in the plot below is shown. Transcript size increases from left to right. Generally, regardless of transcript length, m6A has a cumulative effect on the likelihood of enrichment in stress granules. b, Scatter plot demonstrating the correlation between m6A levels and stress granule enrichment in NIH3T3 cells. The y-axis shows the relative level of enrichment of transcripts in stress granules relative to the cytoplasm. The x-axis shows the length of individual transcripts on a logarithmic scale. The number of m6A sites in each individual transcript is color-coded as shown (yellow=0, light green=1, blue-green=2, blue=3, violet=4 + ). Dotted lines are shown connected to the boxplots in panel a that summarize the scatter data per m6A site. As can be seen, the highly methylated mRNAs of any mRNA length exhibit higher stress granule enrichment.
