Fig. 4: Assembly of the intact NuRD complex modulates the movement of active enhancers.
From: Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD
a, Left, example trajectory of the Nanog enhancer segmented to show periods of slow and fast subdiffusive motion (dark and light blue, respectively). Right, α, Lc, Dapp and norm∥V∥ of the locus extracted from the trajectory shown. b, Boxplots of Dapp calculated for 2D trajectories of loci near the Tbx3 and Nanog enhancers, in the presence and absence of MBD3 (*P = 0.045 and *P = 1 × 10–4 for Tbx3 and Nanog, respectively, two-sided Kolmogorov–Smirnov test). c, Gaussian fitting to the distribution of the α values identifies a single slow and two faster states for enhancer loci (Extended Data Fig. 7). The percentage of subtrajectories of enhancer loci exhibiting slow (S) and fast chromatin motions with either a low (F1) or high (F2) α is shown (*P < 0.01, two-way ANOVA). The numbers of trajectories used in the analysis were 237 (Tbx3 + MBD3) and 287 (Tbx3-MBD3); 546 (Nanog + MBD3) and 229 (Nanog-MBD3). Center line, median; box limits, upper and lower quartiles; whiskers, 95% confidence interval.
