Extended Data Fig. 8: Characterization of the kinetics of R-loop formation and supercoiling loss using various dgRNA-SpCas9 RNPs.
From: Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation
(a-d) Distributions of waiting times for R-loop formation obtained for four different RNPs (blue) are fitted to single-exponential decays (red) to obtain the corresponding average waiting time, <tR-on > (± SEM), for (a) S1_T0 SpCas9, (b) S1_T1 SpCas9, (c) S1_T2 SpCas9 and (d) S2_T0 SpCas9. (e-h) Similarly, the mean time elapsed between R-loop formation and supercoil dissociation, <tSuper-off>, was calculated for (e) S1_T0 SpCas9, (f) S1_T1 SpCas9, (g) S1_T2 SpCas9 and (h) S2_T0 SpCas9. See Supplementary Table 2 for further details.
