Table 1 Cryo-EM data collection, refinement and validation statistics
#1 APC/C–CDH1–UBE2C | #2 APC/C–CDH1–UBE2C–UBE2S | |
|---|---|---|
Data collection and processing | ||
Magnification | 180,000× | 180,000× |
Voltage (kV) | 300 | 300 |
Electron exposure (e− Å−2) | 42 | 42 |
Defocus range (μm) | −0.7 to −3.5 | −0.7 to −3.5 |
Pixel size (Å) | 0.82 | 0.82 |
Symmetry imposed | C1 | C1 |
Initial particle images (no.) | 2,001,820 | 1,894,448 |
Final particle images (no.) | 661,289 | 774,933 |
Map resolution (Å) | 4.0 Å | 3.5 Å |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 3–10 Å | 3–10 Å |
Refinement | ||
Initial model used (PDB code) | 7QE7 | 7QE7 |
Model resolution (Å) | 4.0 Å | 3.5 Å |
FSC threshold | 0.143 | 0.143 |
Model resolution range (Å) | 3–10 Å | 3–10 Å |
Map sharpening B factor (Å2) | −188.3 | −159.6 |
Model composition | ||
Non-hydrogen atoms | 35,965 | 36,038 |
Protein residues | 8,989 | 9,007 |
R. m. s. deviations | ||
Bond lengths (Å) | 0.006 | 0.007 |
Bond angles (°) | 0.766 | 0.792 |
Validation | ||
MolProbity score | 1.70 | 1.81 |
Clashscore | 6.84 | 9.17 |
Ramachandran plot | ||
Favored (%) | 98.19% | 97.89% |
Allowed (%) | 1.81% | 2.12% |
Disallowed (%) | 0.00% | 0.00% |
