Extended Data Fig. 9: Structural comparison between mMymk^WT, mMymk^R107A and mMymk^Y118A.

a, The hydrophilic interaction network in the extracellular leaflet of TMs 4, 5 and 6 between mMymk^WT in blue, mMymk^R107A in green, mMymk^Y118A in salmon. Water molecule and Zinc ion are shown as sphere in white and gray, respectively. b, Structural comparisons among mMymk^WT in blue, mMymk^R107A in green, mMymk^Y118A in salmon and the AlphaFold prediction model of mMymk^WT in gray. The arrow indicates the structural shift of TM5. TMs and related residues are labeled. The PC of mMymk^WT is shown as sticks in yellow. c, The cryo-EM maps of ECL2 in mMymk^WT, mMymk^R107A, and mMymk^Y118A. The entire cryo-EM map of each structure is shown and the map of ECL2 is colored in red.