Extended Data Fig. 10: Ab15G1 binds to the extracellular regions of mMymk.
From: Cryo-EM structures of Myomaker reveal a molecular basis for myoblast fusion
a, Pull-down assay of Ab15G1 or control antibody (anti-Hemophilus influenza type B antibody) with mMymk or mMymk-Fab18G7 complex detected by Coomassie staining. The molecular markers (left) and proteins (right) are indicated. Data shown are representative of two independent experiments. b, Immunofluorescence microscopy of live C2C12 myoblasts expressing GFP (green). Endogenous mMymk is stained with Ab15G1. Scale bar, 10 μm. Experiments were repeated three times with similar results.
