Fig. 5: Targeting to EMC facilitates C-terminal TMD insertion.
From: EMC rectifies the topology of multipass membrane proteins
The GABRA1 TMD4 or SQS TMD was preceded by either the 23L TMD or TMD1–3 of rhodopsin, separated by a 100 amino acid linker. These constructs were translated in the absence (Ø) or presence of SPCs from WT or ΔE cells and analyzed by SDS–PAGE and autoradiography. Substrates with zero, one or two glycans attached are labeled. Per cent C-tail translocation, calculated as in Fig. 4, is indicated.
