Fig. 6: Systematic analysis of multipass membrane protein orientation.
From: EMC rectifies the topology of multipass membrane proteins
a, The topology of all annotated multipass proteins in the human genome (1,784 total), determined using a combination of AlphaFold2 structure prediction to identify TMDs and the positive-inside rule to deduce orientation (see Supplementary Table 1). The C-tail locations (Cexo or Ccyt for exoplasmic and cytosolic, respectively) and lengths were tabulated and plotted versus the numbers of TMDs in the protein. b, The data from a were re-plotted relative to the ΔGapp65 score of the final TMD. Representative substrates analyzed in Fig. 7 for EMC-dependent translocation of their C-terminal TMDs are highlighted in red.
