Extended Data Fig. 8: Modest upregulation of IAPs and reduction in H3K9me3 enrichment in D1+/−, D3a+/− GV oocytes.
From: Auto-suppression of Tet dioxygenases protects the mouse oocyte genome from oxidative demethylation
a, Bisulfite sequencing analyses of an IAP element (accession M17551) in WT and D1+/−, D3a+/− GV oocytes. 200 oocytes were used for each genotype. b, RT-qPCR analysis of IAPs in GV oocytes with the indicated genotypes. 200 oocytes were pooled for each sample. Expression levels were normalized to 18s rRNA, and then compared to WT, which was set to 1.0. Mean and s.e.m. were determined from six biological replicates. c, No significant change in H3K9me3 level at IAPs in oocytes with Dnmt1 and Dnmt3a haploinsufficiency. Shown are H3K9me3 enrichment profiles (CPM) in a region including IAPs and their 2-kb flanking regions (upper panel), and heat maps of H3K9me3 signals (CPM) within 2-kb peak summits at IAPs in WT and D1+/−, D3a+/− GV oocytes (bottom panel). 500 GV oocytes were pooled for analysis.
