Extended Data Fig. 2: Generation of Tet3-LCD conditional knockout mouse model and their phenotypic analysis.
From: Auto-suppression of Tet dioxygenases protects the mouse oocyte genome from oxidative demethylation
a, Extended DataRNA-seq tracks of the Tet3 locus in GV oocytes of WT (blue) and LCD KO (red) mice. The deleted LCD-coding region is indicated by a dashed line box. b, Confirmation of the GFP expression in MII oocytes upon Cre-mediated inversion of the loxP/loxN-flanked region. MII oocytes were obtained 20 hours after IVM. BF, bright field. Scale bar, 100 μm. c, Representative images of 5caC and 5mC immunostaining in GV oocytes. Dashed lines represent cell boundaries (from differential interference contrast view). Scale bar, 20 μm. d, Representative images of ovaries collected from adult CKO female mice and controls. Scale bar, 500 μm. e, Representative images of H&E-stained cross-sections of ovaries from adult CKO female mice and controls. Scale bar, 200 μm. f, Classification of follicles per ovarian section at different ages. Six sections per ovary from three mice per genotype were analyzed. Statistical significance (indicated P value) was analyzed using an unpaired two-tailed Student’s t-test. Data in b and c represent three independent experiments.
